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Transcription factor binding motif analyses of mesenchymal stem cells (MSCs) of different tissue origins.
De novo motif analyses of the Foxa1-bound regions using MEME (Bailey and Elkan, 1994) revealed a substantial enrichment in Foxa motif sites (Fig. 1E).
Separate analysis of the distal subset, which is more similar to the K562 unique peaks, eliminated problems that occur with motif analyses of promoter regions.
Motif analyses of MLL4 binding sites during adipogenesis and myogenesis have identified motifs of additional lineage-determining TFs, such as adipogenic TFs EBFs, GR and NFIC and myogenic TFs TCF3, Runx1 and TEAD4.
Through topology and motif analyses of YrbE homologs, we identified a region within the penultimate cytoplasmic loop that may serve as the site of interaction with the putative cognate Mkl ATPase.
Other transcription factors may play a role in downregulation; for example, motif analyses of the downregulated gene promoters revealed a significant enrichment of transcription factor Sp1 binding sites (data not shown).
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Examination of these binding sites by de novo motif analyses revealed an enrichment of two sequences within ±25 bp of the summits of binding sites (Fig 5Bi and ii; E-value = 8 × 10−92 and 8 × 10−41 for the two motifs shown in i and ii, respectively).
In support of this, de novo motif analyses revealed stronger enrichment of the C/EBP motif at the top-ranked sites.
Besides the results of motif analyses, which might be helpful for prediction of phosphorylation sites in plants, actually, PhosphAt database provided prediction service for Arabidopsis (26).
The results of motif analyses thus suggest that mechanisms underlying the formation of uncapped 5′-ends containing these short motifs might play prominent roles in the production of predominant uncapped 5′-ends in addition to miRNA regulation especially in the 3′ UTR.
MochiView serves as both a motif analysis platform and a feature-rich genome browser, and integrates these features to allow the visualization of motifs across a genome plot and the refinement of motif analyses using data imported by the user into the MochiView database (for example, genome alignments, ChIP data, or expression data).
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Justyna Jupowicz-Kozak
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