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Mothers will complete the pre-, post-, and follow up measurement online.
Participating children and their mothers will complete baseline, post intervention, and a 3-month follow-up assessments (see Figure 1).
In addition, all participating mothers will complete a brief questionnaire delivered on-line or by SMS at 12 and 18 weeks to determine current breastfeeding status.
The mothers will complete multiple questionnaires to assess the baseline characteristics, the child's exposure to environmental factors, maternal pre- and post-natal psychological stress, and the child's neurodevelopment, nutritional status, and development of allergic and respiratory illnesses.
Subjects or their mothers will complete diaries to collect information on meal times, cosmetics and medication use, and urination frequency for input into the physiologically based pharmacokinetic (PBPK) models (described under research area 1, below).
In order to investigate the role of PNMS in influencing the children's developing immune system, mothers will complete a 16-item questionnaire pertaining to their own symptoms of asthma, eczema and allergies, and those of their child and partner.
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At the 2 and 3 year visit, the mother will complete a 24 hour recall for the child.
The father and mother will complete their respective questionnaires, put them in separate envelopes, and seal the envelopes, individually.
At age one and 3 years, the mother will complete a 24-hour dietary recall for the child.
For the eldest child in the RCT, the mother will complete a modified version of the CLASS, which has been validated in children 5 - 6 and 10 - 12 year of age [ 41].
All mothers who will complete all questionnaires will receive about 50 Euros for their participation.
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