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Cells, consisting mostly of macrophages (over 90%), were harvested by peritoneal lavage using 5 ml RPMI medium supplemented with 5% FBS, 4 days after intraperitoneal injection of thioglycolate.
To this effect, we observed that the adoptive transfer of a population composed mostly of macrophages but not DCs from WT mice was sufficient to reduce mortality induced by CB4 in TLR3KO mice by preventing viral-mediated cardiac pathology.
Infiltration of inflammatory cells was also present and consisted mostly of macrophages, lymphocytes, plasma cells and individual granulocytes.
The TNF-α immunopositive cells in the mucosa comprised mostly of macrophages and occasionally lymphocytes in the lamina propria.
Skin lesions were mostly characterized by mild to moderate deposition of amorphous granular calcium material, positive to Von Kossa stain, and by mild to moderate inflammations composed mostly of macrophages, lymphocytes, and few giant cells.
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In normal and benign tissues, leucocytes (reactive with 2D1) found predominantly in the lamina propria, comprised T cells mainly of the helper/inducer (OKT4) subset, DR + cells in approx. equivalent proportion and a few OKM1+ cells mostly of macrophage morphology.
In addition, increased NEMO expression was also found in the lymphoid tissues, including lymph nodes and thymus, but not in the peritoneal cavity, which was mostly comprised of macrophages (Supplementary Figures S3B S3F).
In agreement with previous observations (Avellino et al., 1995; Jurecka et al., 1975), we found that the bridge was mostly composed of macrophages (50%) and neutrophils (24%) with fibroblasts (13%) and endothelial cells (ECs) (5%) as minor components.
During the first weeks of development, the zebrafish larva has no functional adaptive immune system, relying mostly on macrophages and neutrophils, to fight infections (Lieschke et al., 2001; Meijer and Spaink, 2011; Renshaw and Trede, 2012).
All treated animals (and one control) had degeneration of cardiomyocytes with subacute inflammation consisting of mostly macrophages.
Through microscopic morphologic analysis of the cells, the populations harvested from the canine peritoneum was found to be composed of mostly macrophages, monocytes and neutrophils (Fig. 2).
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