The intracellular SUMO-1-peptide fusion is mostly nuclear, probably as consequence of the nucleic acid binding properties of the Tat PTD but also possibly due to the SUMO-1 domain.
GR expression in TNBC single-tissue samples and TMA cores was characterized as mostly nuclear, with some concurrent cytoplasmic reactivity.
GR expression in TNBC single-tissue samples and TMA cores was characterized as mostly nuclear, but sometimes with concurrent cytoplasmic reactivity.
Experimentally, FOXO localization is usually measured as the fraction of cells having FOXO mostly nuclear, mostly cytoplasmic or both nuclear and cytoplasmic, as determined with fluorescent-labelled FOXO.
Unlike some other APOBEC3 proteins such as human APOBEC3B, which is mostly nuclear, both human APOBEC3G and pig APOBEC3F appeared predominantly cytoplasmic in either the human or the pig cell lines (Figure 3; [31], [41], [41]).
In the current model, the fraction of FOXO in the nuclear or cytoplasmic compartment will be treated as equivalent to the experimentally determined fraction of cells having FOXO mostly nuclear or cytoplasmic.
As expected, endogenous MDM2 labeling in the PIR-H1299 cells was relatively faint and mostly nuclear while PIR was mainly localized to the cytoplasm (Figure 2a).
In controls with unperturbed levels of PKAcα, HSF1 was mostly nuclear and after heat shock localized to stress granules in the nucleus (Fig. 3A, B) as described above [24], [41].
As shown in Figure 1C, HA-Yki was mostly cytoplasmic in S2R+ cells without ectopic Ncoa6, but was mostly nuclear in the presence of ectopic Ncoa6, and an Ncoa6 protein with PPxY mutations had no effect on HA-Yki localization.
