Gene expression analysis revealed transcriptional alterations, which are mostly cell line specific.
The effects are mostly cell line specific and have an influence at the outcome of the treatment.
While engineered SiO2 nanoparticle toxicity is being widely investigated, mostly on cell lines or in acute animal experiments, the practical importance of as well as the theoretical interest in industrial condensation aerosols with a high SiO2 particle content seems to be neglected.
Transcriptional responses of butyrate were studied mostly in cell lines [14], [15], [21] [32] and some studies were performed in animals and human patients [3], [30], [33] [35].
Both mostly sensitive cell lines showed reduced expression of IAPs with the lowest levels of all IAPs in Capan1 cells.
The panel consisted of mostly cancer cell lines but also included human foreskin fibroblast (HFF) and human embryonic HES-2 cells.
The sets of genes expressed in human normal liver, HCC and HepG2 cells, glucose, intracellular pathways, or transcription factors modulated by various stimuli were obtained exclusively with human cells, mostly cancer cell lines including HepG2 cells (Table 1).
However, these latter studies were conducted mostly with cell lines in vitro and have not been validated by in vivo approaches.
In the lowest (simplest) tier of assessments, evaluations are expected to primarily rely on the results of high- and medium-throughput in vitro screening tests in mostly human cell lines, as well as complementary in silico predictive methods.
Importantly, the current data for IFN-β and IFN-λ gene expression is entirely based on in vitro studies mostly utilizing cell lines or gene-deficient murine embryonic fibroblasts (MEFs) with model viruses that are not important human pathogens.
