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Some mutant and deficiency stocks were generated in our lab, others were gifts from other investigators, but most were obtained directly from the Bloomington stock center.
Isolates were obtained from 6 districts within Uganda (Table 1 and Figure 1), although most were obtained from patients in Kampala.
All midline pediatric HGA samples were from needle biopsies (pons) or partial resections (thalamus, spine, cerebellum) and most were obtained prior radio- and/or chemotherapy.
The mutation status of the patients in this trial was based solely on a single sample of LGSC; most were obtained from the primary tumor and a small percentage were obtained from the recurrent tumor.
Clade 1 basal lineages were sourced from the south central part of the island and most were obtained from P. mooreana and P. tohiveana, both members of the P. suturalis species complex [ 23].
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Most are obtained from clinic-based cohorts where rates range from 3%to28%8% [ 12, 20- 23].
Most isolates were obtained from travelers or immigrants, most of whom were infected in Africa and Asia.
Macroremains were rare (except for Aurélie lake), and most dates were obtained from bulk sediments (gyttja).
Most parameters were obtained from experiments, although some were taken from the literature.
In the end, however, most interviews were obtained at public places.
The method accuracy was assessed and good recovery values (>70%, in most cases) were obtained.
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