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Taken together, our results suggest a scenario in which Gli proteins require SUMOylation in order to achieve the highest levels of activity necessary to induce the most ventral cell identities within the neural tube.
By 100 hph, Ptch1 protein and mRNA were barely detectable within the most ventral cell types.
Unexpectedly, the application of melatonin elicited enhanced neuronal activity in the most ventral cell of the cholinergic cluster.
First, the induction of most ventral cell types can take place in the absence of Shh signalling, as long as the repressive activity of Gli3 is removed.
Using gain- and loss-of-function assays, we show that Notch is required for cells to acquire the most ventral cell fate in response to Shh but that attenuation of Notch signalling is equally important for these cells to fully differentiate as floor plate.
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The ventral tegmental (VTA) height was determined by drawing a vertical line between the most dorsal and most ventral cells in the VTA.
Although the higher levels of Tg(GBS-GFP) activity appeared to persist for a somewhat longer time in the most ventral cells of the neural tube in Pax6 Olig2 mutants, the range of Tg(GBS-GFP) activity was unchanged.
While Pax7-positive and differentiating dermomyotome cells are absent in the central domain in fss/tbx6 mutants at the end of segmentation, the most dorsal and most ventral dermomyotome cells are present in approximately normal numbers and differentiate at percentages similar to wild type (Fig. 3B).
However, the relative proximity of these Wg-expressing disc cells to the ASP decreased during the L3 period: although the most ventral Wg-expressing cells were within 5 10 μm of the ASP in early L3 discs, there were no Wg-expressing cells within 60 70 μm in late L3 discs.
Putative Renshaw cells labeled with the virus usually formed small clusters at the most ventral edge of the MN pool (Fig. 6A C), had cell bodies 10 20 µm in diameter and expressed the calcium binding protein calbindin-D28K (Fig. 6B; [29]).
Within the ventral hippocampus, all rats showed some partial sparing of the cell layers of the lateral blade of the dentate gyrus, as well as the most ventral part of CA1 and CA3.
Related(18)
most anterior cell
most ventral cells
most ventral edge
most ventral arborization
most ventral point
most ventral recording
most ventral tip
most ventral contact
most ventral seed
most ventral prototroch
most ventral expression
most ventral portion
most ventral floor
most ventral position
most ventral midbrain
most ventral region
most ventral part
most ventral extension
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