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This was accomplished by calculating perfusion levels in the entire tumor, as well as in the most vascularized part of the tumor, and then correlating those values with the surgical pathology [ 35].
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Microvascular density was determined by manually counting CD31-positive vessels in 5∼15 most vascularized areas of each section under 400× magnification as described (Weidner and Folkman, 1996).
The area of a tumor to be imaged was determined according to 3 conditions as reported previously [ 27, 29]: (a) the whole tumor, if possible, should be seen on the image; (b) the sectional plane should contain the solid part (wall, septa, and papillae) of the tumor; and (c) the most vascularized area was selected.
Gliomas are among the most vascularized tumors.
GBM represents one of the most vascularized tumors [ 20].
The choroid covers the outer retina and is among the most vascularized tissues in the body.
In human placenta, the most vascularized organ, the presence of netrins has also been reported.
The sections were scanned at low power (100×) to select the most vascularized (hot-spots) areas.
Pancreatic cancer represents one of the most vascularized and angiogenic solid tumors [ 13].
This technique quantified the most vascularized tumour areas, the so called "hot spots" of the whole tumour.
The effect of pazopanib on HCC, one of the most vascularized tumors, has not been reported before.
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