For high reliability, the RT-PCRs are designed such that every genome segment is divided into two amplicons and for the most variable segments redundancy is included.
As evident from figures 3 and 5, the N and C terminii along with BC and DE loops are the most variable segments both in terms of length and composition within the ferritin family.
Furthermore, J5/4 is one of the most variable segments as deduced from the Naegleria GIR1 sequence alignment albeit with conserved features (Wikmark et al, 2006).
The complementarity determining regions (CDRs) of an antibody are the most variable segments of the variable domain that are essential for the antibody-antigen binding specificity and affinity [ 14].
However, the four amino acids following the EPIYA motif were most variable in segment A (Table S7).
Seg-2/VP2 are also the most variable genome segment and protein (>45.8% nt and >31.1% aa identity), reflecting the evolution of several distinct serotypes within each Orbivirus species/serogroup, and the separation of strains from different geographic origins, into distinct topotypes within these serotypes [2], [9], [15], [78], [79], [82] [84].
It is the most variable genome segment of SBV and related viruses (7, 9, 10 ).
We included the most variable mtDNA segment, the hypervariable region 1 (HVR1) of the control region since it has a high rate of nucleotide change, allowing recently diverged lineages to be distinguished [ 19- 21].
A high turnover of virus types between years was detected in each vole population (Fig. 5, Figure S2), yet a few virus sequence types persisted during the eight years of the study even for the most variable M segment, despite the high evolutionary rate of hantaviruses.
As shown in Figure 1, the S7 segment is the most variable among the Dendroaspis toxins and may result in functional diversification due to accelerated exchange.
