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Thus, the BI/BII ratio reflects the most variable DNA helicoidal descriptors.
Consistent with this observation, unsupervised hierarchical clustering of the most variable DNA methylation and gene expression across all samples revealed clustering of matching primary and xenograft samples.
First, we performed unsupervised hierarchical clustering of the 10,000 most variable DNA methylation loci and observed two distinct clusters of methylation profiles among DCIS samples.
These positions reside in the α-helix domain, close to the most variable DNA recognition positions, i.e. positions −1 and 6.
SSRs are among the most variable DNA sequences in the genome [ 19], and the mutation rate and type depends mainly on the number of repeat motifs [ 20].
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Similar to LCLs, the average DNA methylation levels varied in the different amplicons analyzed: Highest (and most variable) average DNA methylation was observed in A3 (average 85%), whereas CpG sites in the amplicon A-5 located directly upstream of the transcriptional start site was much less methylated (average 2%, Figure 4a).
Long, promoter-associated CGIs are stably hypomethylated across different cell types, while short, intra- or inter-genic CGIs exhibit the most variable patterns DNA methylation.
Although mitochondrial DNA is often considered to evolve very slowly in corals, and not to be informative for intra-specific comparisons [24], [25], [26] the mitochondrial control region was the most variable of the three DNA regions studied here.
Using DNA methylation of different ReDS revealed that tissue to tissue DNA methylation was most variable at L1, non-islands and weak CpG islands and that the pattern of DNA methylation across tissues was similar at these three ReDS.
First, a pilot study was performed to find the most variable sequences among 17 plastid DNA regions previously used in phylogenetic and phylogeographic analyses [see 30 for details].
The oligonucleotide sequences were designed inside the HCDR3 regions, since they represent the most variable (thus, selective, in terms of DNA sequence) regions in the antibody repertoire.
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