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The most useful transcript sequences are derived from high quality full-length cDNA sequences which contain the complete transcript in a single clone [1].
The most useful transcript sequences are derived from high quality full-length sequencing of inserts from cDNA clones (FLIcs) that contain the complete protein coding sequence (cCDS).
The most useful transcript sequences are derived by complete insert sequencing of clones containing the entire length, or at least the full protein coding sequence (CDS) portion, of the source mRNA.
High quality sequenced FLICs represent the most useful transcript sequences, and this has led to large scale sequencing of full length insert cDNAs in several non-model species (e.g.[ 21, 23- 26]).
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This type of further work would be most useful if done in a way that identified transcript 5' ends (potential transcription start sites) genome-wide, which is possible using RNA-seq approaches [ 122].
However, in practice such a system would be most useful in spotting individual words rather than creating reliable transcripts, he said.
One of most useful features of RNA-seq analysis is that it allows direct and quite accurate estimations of transcript levels.
In particular, molecular monitoring of BCR-ABL transcript levels by real-time quantitative PCR (RQ PCR) is progressively becoming the most useful and precise way to monitor CML patients.
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