Probes corresponding to 16,949 genes were obtained with the most updated annotation file [ 14].
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We identified a total of 404 orthologous genes present in all 58 genomes (see additional file 2), a gene set defined here as the most updated cyanobacterial "core".
To provide the most updated bufonid phylogeny for placing our focal taxa, we included GenBank data of representatives of all available unique bufonids (234 species, 39 genera) and several outgroups (seven species) (Additional file 2).
A full T. castaneum P450 gene list with the most updated assembly and annotation information including clan, name, accession number, symbol synonyms, map position and amino acid length are provided in the Additional file 1[ 34].
