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The transformants were mitotically stable, and most transformants were estimated to have a single copy of the T-DNA integrated into the genome (Figure 1B).

Because N. crassa is multinucleate, most transformants are heterokaryons.

Most transformants are resistant to drugs for other (unknown) reasons [ 13].

That these cells carry the dnaE(Ts) mutation was controlled by transferring the EmR region into the 168 strain and showing that most of the transformants were Ts.

Most of the resulting transformants were heterozygote for intact and inactivated crtI.

In most cases, positive yeast transformants were streaked out for single colonies, these were expanded on quarter petri dishes, and yeast genomic DNA was isolated [ 34].

The phenotypic responses of the transformants were most pronounced in mature tobacco plants, where XS plants were stunted, possessed thick, dark leaves, and exhibited reduced flowering and root formation, and XAS plants were taller and had increased numbers of inflorescences and flowers.

The plant transformation was performed as described previously59, and the transformants were selected for kanamycin resistance on MS medium.

Transformants were selected on kanamycin-containing LB agar plates.

The transformants were selected on the basis of green fluorescence, total genomic DNA (gDNA) was extracted from these fluorescent transformants.

The transformants were cultured and selected.