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To facilitate comparisons, the statistically-significant salt-induced or repressed genes were analyzed between the three most sensitive and three most tolerant genotypes, excluding L. filicaulis with intermediate tolerance.
Out of 300 genotypes, we picked 142 RILs that comprised the 7 most tolerant genotypes (SES score 1 3) and 34 most sensitive ones (SES score 9), and the rest (101) were random genotypes with intermediate to sensitive SES score (5 7).
In particular, after 48 h of treatment, Carnaroli, Gigante Vercelli, Loto, Maratelli and Vialone Nano resulted to be the most sensitive cultivars, showing both the lowest RWC (<15 %) and the highest EL (>94%%) values, whereas Augusto and Eurosis resulted to be the most tolerant genotypes, showing both the highest RWC values (>80 %) and the lowest EL values (<40 %).
During the first four trials, plant survival, elongation and tillering ability under SF were found to be effective in selecting the most tolerant genotypes.
From the six trials conducted during this study, it became clear that severe stress imposed early during the vegetative stage, i.e. increasing the floodwater depth of 20 30-day-old seedlings to reach 50–60 cm within 20 30-day-oldowed by maintaining that depth through maturity, was effective in identifying the most tolerant genotypes.
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Similarly, in our research and through the entire experiment, the most tolerant genotype produced 10.6 30.15% grains more than the most susceptible genotype under constant stress exposure and periodic stress exposure, respectively.
FR13A, the most tolerant genotype, had the slowest underwater elongation, followed by IR49830 and the other Sub1 introgression lines.
It was proposed that the most tolerant genotype used in the experiment of Wissuwa et al. (2005) preferably mobilized P to the roots, which would increase P concentrations and consequently promote growth.
Only 4% of salt-induced genes in the three most-sensitive genotypes were common to all three, while 7% of salt-induced genes in the three most-tolerant genotypes were common to all three.
In order to find the most tolerant and/or competitive genotypes in the Great Basin, we recommend wide-scale collections be undertaken from heavily invaded areas, as this and other studies have shown that these populations may be evolving in response to B. tectorum.
The differences in pollen grain development among the tolerant genotypes are most critical factors to determine fruit set under heat stress.
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