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Most tissue clusters display evolutionary and functional relatedness.
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In most tissue types, mutations predominantly cluster within the three aforementioned hotspots: E542K, E545K and H1047R.
However, focusing on the acetylated proteins that are specifically enriched for each of the tissue clusters identifies tissue-specific pathways, which for the most part have not previously been shown to involve lysine acetylation.
Two-way hierarchical clustering identified expected tissue clusters in accordance with tissue type and a number of cDNA clusters having similar gene expression patterns across tissues.
Because most genes are not expressed in most tissues, this prevents the clustering algorithm from seizing on noise amplified in the log scale.
Most tissues with the same embryonic origin clustered together in the NJ-tree.
Genes associated with Cluster II exhibit the most tissue-specific patterns of gene expression (Fig. 5A).
Considering tissue clustering, fetal brain and testis separate most from the other tissues.
Above the 3 8% oxygen concentration typical of most tissues, we find that cancer cells depend on high levels of the iron sulfur cluster biosynthetic enzyme NFS1.
In mammals, housekeeping genes form clusters [ 21, 22], as do low-expression genes that are inactive in most tissues [ 23].
The dimensionality reduction analyses suggest that there are differences between tissue types, such that the expression profiles of NAT are closer to the tumor cluster in some tissues and closer to the healthy tissue cluster in others.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com