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Antimycin is the most tightly bound of the three inhibitors, and binds stoichiometrically to the isolated enzymes from all three species under the cytochrome c reductase assay conditions.
The x-rays would rip the most tightly bound electron out of an atom, leaving hosts of atoms in highly energetic states.
It is however affected by resistance mutations, whereby the T315I mutant of Abl is most tightly bound (ca 10 nM), and the gatekeeper+2 mutations F317I or F317L are most weakly inhibited (3000 and 600 nM, respectively).
It was determined from the isothermal titration calorimetry (ITC) data that Mg+2 and Mn+2 are most tightly bound to PP i, Kb = 41,000 ± 2000 M-1 and 28,000 ± 50,000 M-1 respectively, and UTP, Kb = 14,300 ± 700 M-1 and 13,000 ± 2,000 M-1 respectively.
Palmitic acid is the most tightly bound FA to the β-lg structure.
FRAP analysis of GFP-tagged subtypes in cell-based assays showed that H1.4 and H1.5 are most tightly bound to chromatin, whereas H1.0 and H1.3 have intermediate binding and H1.1 and H1.2 are weakly bound.
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Oxalate and carbonate remove mainly pectins and a small portion of hemicellulose from the walls, and the following 1M and 4M KOH treatments extract most of the tightly bound hemicelluloses and pectin.
Most intracellular copper is tightly bound and regulated by copper-binding proteins [ 56], and intracellular free copper is essentially undetectable [ 57].
Unlike most nucleoplasmic proteins, factors tightly bound to chromatin and/or DNA tend to be resistant to extraction with detergents or increasing salt concentrations, characteristics that have been the basis for cellular fractionation (or "chromatin fractionation") experiments (21, 22).
Most biological zinc ions are tightly bound to proteins, functioning as catalytic or structural cofactors.
Another problem using serum testosterone levels to gauge androgenic effects at the tissue level is that most of the circulating testosterone is tightly bound to SHBG, while only the free hormone is bioactive.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com