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Although most terminal neurites innervate single Merkel cells, the Gaussian distribution of these connections suggests that they are formed through probabilistic rather than deterministic mechanisms.
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Consistent with the effect of the terminal segments that induced their retraction, the order at which the curve peaked was lower with MPP+, so this was the order at which the values became zero; this means the most abundant terminal neurites were located at order 2, and there were no dendrites at order 6.
Most Merkel cells were arranged individually on terminal neurites (70%, N = 165, Figure 2B,C), although chains of three or more Merkel cells along individual neurites were occasionally observed.
Similarly, ≥80% of terminal neurites were occupied by Merkel cells in most touch domes.
(A ) Distribution of terminal neurites per heminode (N = 219 neurites).
As with terminal neurites, the number of Merkel cell neurite complexes per heminode was broadly distributed.
The distribution of Merkel cells to terminal neurites was fitted with a Gaussian distribution (R > 0.99).
(C ) Confocal projection of six terminal neurites contacting individual Merkel cells (asterisks).
The number of terminal neurites emanating from each heminode was broadly distributed.
To determine how these complexes are arranged within an afferent's arbor, we analyzed the distribution of Merkel cell neurite complexes among terminal neurites and heminodes.
(I ) More than 80% of Merkel cells were contacted by neurites and a similar proportion of terminal neurites contacted Merkel cells (N = 15 touch domes from five mice in G – I ).
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