Sentence examples for most terminal BACs from inspiring English sources

Our method was analogous to performing linkage analysis with the two most terminal BACs in the contigs.

First, 19 of the largest contigs were anchored to the microsatellite-based genetic linkage map [ 2] using markers developed from the two most terminal BACs in each contig (Table 2 and Table 3).

Finally, although not contiguous with the other Gm13 BAC probes, Gm13-BAC-A is one of the most 5′ terminal BACs available for the Gm13 pseudomolecule; in TL-4, this probe also hybridized very near to the signal for Gm04-BAC-2, on the longer arm of the mutant Gm04.

Because the most terminal Gm04 BAC tested (Gm04-BAC-2) is positioned at 44.1 Mb of the 49.2 Mb G. max W82 pseudomolecule, and also due to the lack of more centromere-proximal Gm13-LA probes, our analysis does not rule out the possibility of a reciprocal translocation in which a small fragment of Gm04 was transferred to the truncated TL-4 Gm13-LA.

Based on cytological FISH results, HSY BAC SH52H15 of Knob 4 mapped to the most terminal position compared to the other four knobs at metaphase I. Authors of that report suggested that the centromere of the Yh chromosome is located between Knobs 4 and 5 [ 16].

Two terminal BACs from each end of the largest 4608 agarose contigs (four BACs per contig, totaling 18,432 BACs) from the preliminary assembly were fingerprinted using HICF.

Contigs were first assembled by BES alignment to the chicken genome and then merged using terminal overgo hybridization to a common BAC or common placement of terminal BACs in a single fingerprint contig.

FISH mapping with Gm01 terminal BACs indicated Gm01 to be intact in TL-5 (data not shown).

3 Informative meioses No linkage to markers on the current genetic map 1 Percent recombination based on two-point linkage 2 likelihoodlihood ratio of linkage vs. non-linkage 3 No linkage to markers on the current genetic map * When a polymorphic marker was not available (n/a) for a terminal BAC clone, this most distal mapped clone was used for linkage analysis.

The terminal BAC clones of each contig, or clones at potentially weak points (resulting from "Ends-to-Ends" assembly) were chosen for linkage mapping.

To circumvent this, if two agarose contig-terminal BACs were suggested to be joined by HICF, we lowered the cutoff value for joining agarose contig-terminal BACs by two denary intervals, e. g. when the overall cutoff was set to 1e-12, woulduld accept an overlap at the cutoff at 1e-10 if the two BACs were found in the same HICF contig.