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This tenet is challenged by the observation that most suppressor mutations are not close in space to the initial mutations [69], while coevolving sites are most often spatially clustered [53], [54], [68], [70].
Most suppressor mutations have not been mapped genetically.
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Most of these suppressor mutations map to the inner surface of the cavity formed by the RT Thumb, Linker, Endonuclease and RNaseH-like domains [ 9 ].
Our results suggest that most EMS-treated bal individual plants carry alleles that suppress bal phenotypes and that these alleles are revertant alleles, intragenic suppressor mutations, or dominant extragenic suppressor mutations tightly linked to the bal allele.
Suppressor mutations may occur within the gene carrying the primary mutation (intragenic suppressors) or in another gene, generating a phenotype that counterbalances the effect of the primary mutation.
Suppressor mutations are secondary mutations which restore the original phenotype in an organism that has undergone a primary mutation.
To do this we fractionated our results for the total yield of reversion mutations into those due to true back mutations and those due to suppressor mutations.
When we did this we found that the 1-hit mutations were due to true back mutants, and the 2-hit mutations were due to suppressor mutations.
Huang, B., Ramanis, Z. & Luck, D.J. Suppressor mutations in Chlamydomonas reveal a regulatory mechanism for flagellar function.
Strains that can grow on 5-FOA medium were expected to have suppressor mutations of rrn5 disruption.
In this scenario, the H101Rand A98P mutations would represent suppressor mutations.
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