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Model jumping between fixed-rate amino acid models was used to determine the most suitable substitution model.
Gene genealogies were constructed in PAUP using ML with the HKY and Jukes Cantor as most suitable substitution models for msh1 and 18S, respectively.
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Model jumping between fixed-rate amino acid models was used to determine the most-suitable substitution model, otherwise using default parameters.
Jackson Bird would be the most suitable replacement.
Using MRMODELTEST 2.2 [ 106] we determined that the GTR model of nucleotide substitution with gamma distributed rate variation among sites and nine rate categories (GTR+Γ) was the most suitable nucleotide substitution model for our full sequence set.
The most suitable nucleotide substitution model for each of the gene regions was selected in jModeltest ver. 0.1.1 (Posada [2008]) using Akaike information criterion (AIC).
As described above, we determined that the HYK+I model was the most suitable nucleotide substitution model for our 48 sequences; and using BF, we determined that the sequences were behaving in accordance with a strict molecular clock rather than a relaxed clock, and that a constant population size prior had a better fit than an exponential growth prior.
We used Akaike information criterion and jModelTest version 0.1 (Posada 2008) to identify the HKY+G model as the most suitable model of DNA substitution for the Cytb data and the TrN model for the RpS7 data.
For the distance-based approach, MODELTEST 3.06 (26 ) was used to establish the most suitable model of DNA substitution that best fitted our dataset, and a phylogenetic tree was obtained by using the neighbor-joining algorithm.
Our data indicate that among the peptides tested, the BN5/BN8 pair with the NMeGly11 and Sta13-Leu14 substitutions would be the most suitable compound for rapid high-contrast GRP-R targeting for imaging applications in vivo.
The HKY + I + G model with 6 substitution categories was determined to be the most suitable model by Modeltest and was used for all subsequent nucleotide analyses.
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