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Yeast extract is the most suitable nitrogen source for AR80 activity.
Taking all factors into consideration, YE and CSL were chosen as the most suitable nitrogen sources for M. alpina scale-up culture, among the nitrogen sources tested.
The optimum concentration of the most suitable nitrogen source was assessed by performing incubation experiments with culture media containing different concentrations of that nitrogen source.
These results show that yeast extract is the most suitable nitrogen source for stimulating AR80 activity, so yeast extract was used as the nitrogen source in the subsequent experiments.
As Fig. 1d shows, tryptone was the most suitable nitrogen source for SrtA expression, which gave the highest values of cell biomass and enzymatic activity (1407.1 U/mg DCW).
These results are in accordance with Hou et al. [ 5], who demonstrated that the most suitable nitrogen sources for laccase production by P. ostreatus (strain 32) were peptone and yeast extract, in comparison with urea, ammonium sulfate, and ammonium tartrate.
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In the flasks, the results showed yeast extract or corn steep liquor were the most suitable sole nitrogen sources for biomass and ARA yield.
Among the variables of medium components, glucose, yeast extract, and MgSO4 were identified to be the most suitable carbon, nitrogen, and mineral sources, respectively.
Starting with enzyme production medium, glycerol and yeast extract combined with NaNO3 were screened to be the most suitable carbon and nitrogen source, respectively.
The previous data indicated that the most suitable carbon source, nitrogen source, salt and vitamin were glucose, yeast extract, KH2PO4 and Ca D-Pantothenate, respectively.
The most suitable carbon source, nitrogen source, and culture temperature were agar, yeast extract, and 25 °C, respectively, for agarase production by one-factor-at-a-time design.
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