The show's most successful sequences, which come after the intermission, are the simplest in concept, managing to turn the minimal staging into an advantage.
One of the most successful sequence-based methods is CRpred [ 27], which used several types of sequence-based features including position-specific scoring matrix and entropy of weighted observed percentages extracted from multiple sequence alignment using PSI-BLAST [ 28].
Behind it all, there is Mad Men, which probably has the most successful title sequence of the modern era – if, admittedly, one that borrows, evidently, from the work of the legendary Bass.
This is illustrated by what was commonly regarded as one of the most theatrically successful sequences in Daisey's show – one that also turns out to be untrue.
Substantial improvements in both read length and throughput have been achieved since its introduction, enabling reversible dye termination SBS to become the most successful massively parallel sequencing platforms.
HAL's design was guided by two observations: (i) breakpoint graphs are the most natural way of representing genome rearrangements (Pevzner and Tesler, 2003) and (ii) progressive alignment (based on a phylogenetic decomposition) has been the most successful heuristic for multiple sequence alignment (Notredame, 2007) and is likely to remain so for whole-genome alignment.
One of the most successful applications for exome sequencing and related techniques to date has been the detection of copy number variants.
(By the way, if you're wondering what Sequence does for one of the biggest but also most tight-lipped companies in the world, you won't get an answer: "We're proud to work closely with one of the most successful companies of our generation," Sequence notes.
The most successful application of single-cell sequencing to date relied on microfluidic cell separation and resulted in the sequencing of at least 1500 genes from a representative of the previously uncharacterized TM7 lineage (Marcy et al., 2007).
As production of Stx2 is directly linked to phage replication [ 50], it is not surprising that we were most successful in isolating phDNA for sequencing from lineage I isolates.
This almost Puritanical suspicion of theatrical enchantment for its own sake keeps "Mary Poppins" from ever achieving the undiluted wonder of, say, the opening sequence of that most successful of Disney Broadway ventures, "The Lion King".
