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In liver tissue, most subunits of these macrocomplexes were identified.
Since most subunits of the respiratory chain complexes-and all subunits of complex II-are encoded in the nucleus, but several key proteins are still encoded by mitochondrial DNA (mtDNA), a decreased expression of all OXPHOS complexes except for complex II is not difficult to envision, but the consequences are far reaching.
For example, both Plasmodium and Toxoplasma seem to lack most subunits of the ATG1 initiation complex.
As expected for a ubiquitous cell machinery complex, the expression of most subunits of the 26S proteasome is detected by microarrays in oocytes and hESC.
First, according to the principle, reducing the expression of most subunits of a protein complex will down-regulate the activity of that complex.
It is also clear from the global data in yeast that most subunits of most protein complexes can be overexpressed with no observable phenotypic consequence under laboratory conditions [ 32- 34].
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The existence of multiple splice variants and post-translational modification of most subunits increases the variety of integrins (2).
We did not observe a reduction in the majority of subunits in the complex, suggesting that the levels and stability of most subunits are maintained in the absence of Hdac6 (consistent with Western blotting data in Figure 3B and Figure 5 figure supplement 1).
Despite the self-assembly properties of most subunits it is clear that ESCRT-III filaments require a defined order of subunit assembly.
This is still true when considering protein complexes that are performing an essential function in these conditions, and for those where reducing the expression of most subunits is lethal [ 33].
These results strongly suggest that induction of TAF9B upon neuronal differentiation is specific and distinct from regulated expression of most other subunits of the PIC.
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