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The multi-protein complex structures from which most subunit atomic models are derived are represented on the right.
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We tested two different numbers of GDFs, 8 and 16, for subunit atomic models.
We next performed the fitting calculation, in this case, fitting subunit atomic models into a simulated density map generated from a known atomic model of a complex structure.
To begin with, we describe notations of GMMs of the complex image and the subunit atomic models.
The colores program is able to superimpose a subunit atomic model into a part of the density map, and to output multiple candidate configurations for the subunit.
Finally, the atomic model of the complex structure is obtained by transforming the subunit atomic models, with the optimal positions and orientations obtained by the fitting calculation driven by the GMMs.
Forty-five GDFs were used for the density map of the complex GroEL/ES, and eight functions were used for each subunit atomic model.
In liver tissue, most subunits of these macrocomplexes were identified.
As a more realistic test, we tried to build an atomic model of the GroEL/ES complex by fitting 21-subunit atomic models into the 3D density map obtained by cryoelectron microscopy using the C7 symmetric restraints.
As a more realistic test, we tried to build an atomic model of the GroEL/ES complex by fitting 21-subunit atomic models into the 3D density map obtained by cryo-EM, using C7 symmetric restraints.
For many years, most atomic clocks used atoms excited by microwave signals.
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