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Coating 1 was used in most subsequent experiments, coating 2 was only used to study the effects of protein on antimicrobial activity.

Also, KLK6 promoted cell survival at both 24 and 72 hr time points such that most subsequent experiments were carried out to 24 hr.

We therefore used this paradigm for most subsequent experiments.

In contrast, cells in which Bem1-CAAX replaced Bem1 were fully viable and grew well at all temperatures, so in most subsequent experiments we used Bem1-CAAX.

With the e2 peptide, the sera obtained from both rabbits gave indistinguishable results in all immunofluorescence and Western blot experiments where they were initially compared, and most subsequent experiments were thus carried out with only one of the two.

Similar(55)

The data were also analysed to determine the agonist concentrations that would be most suitable for subsequent experiments involving the addition of selective NMDAR antagonists.

Therefore anti-FLAG antibody was used for most of our subsequent experiments.

Most of the subsequent experiments in this study were performed with the 25 S-Δ7-DA, the more potent isoform (Mahanti et al. 2014; Motola et al. 2006).

We used the most phosphospecific TRAP in subsequent experiments.

The library screen identified 164 antioxidant molecules, which were refined to the 9 most promising molecules in subsequent experiments.

Based on these initial transduction experiments, most subsequent transductions were performed at 10 U/ml for mouse and 9.5 × 10 U/ml for all other species (birds, fish, fly) to achieve similar colony forming unit levels as starting points for our analyses.

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