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Upon biochemical fractionation of rat hippocampal tissues, we found STIM1 to be present in most subcellular fractions, including postsynaptic densities (Figure 1B).
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Most data presented for subcellular fractions of RNA are based on cell lines [ 20, 23].
Most high-throughput mass spectrometry-based proteomic studies of subcellular fractions tend to include proteins from other subcellular compartments, due to some degree of cell lysis (Rey et al., 2005a).
Few reported 14-3-3-binding 14-3-3-binding 14-3-3-binding 14-3-3-bindingasitesithaveosphoproteomic studies of cell lysates and subcellular fractions, where phosphoSer-Pro is the most commonly reported phosphorylation motif overall [ 23].
To establish the subcellular localization of CII after uptake, macrophages were subjected to subcellular fractionation by Percoll density gradient centrifugation, and subcellular fractions were analyzed for markers characteristic of different subcellular compartments.
Subcellular fractions were obtained using the Subcellular Protein Fractionation Kit or the NE-PER Nuclear and Cytoplasmic Extraction Kit (Pierce Thermo Scientific) according to the manufacturer's instructions.
Subcellular fractions were prepared by differential ultracentrifugation.
To isolate iISGs and mISGs, the first step, the enrichment of subcellular fractions, was performed.
Samples can be enriched for selected subcellular fractions or for specific type of proteins (based on post-translational modifications).
Investigations of reactive intermediate formation in subcellular fractions with the use of radiolabeled reagents are also discussed.
4. Subcellular fractions versus Whole cell fraction.
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