Interestingly, the most strongly induced genes in WT were found among this group; 37 out of 104 SAK1-dependent genes were among the top 10% most strongly induced genes (Table 5).
The quantitative real-time RT-PCR analysis of the expression of the three genes (MTT1, MTT3 and MTT5) shows that MTT5 is the most strongly induced (i.e. shows the highest fold-induction) and MTT3 the least induced by diverse environmental stressors (Cd, Pb, Cd+Cu, As, and acid pH).
UNLABELLED: The promoter most strongly induced upon activation of the Cpx two-component envelope stress response is the cpxP promoter.
Genes most strongly induced at 4 h upon phagocytosis (cluster 1) are involved in pathogenesis and associated with the formation of hyphae, including four of the eight genes involved in the core filamentation response17 (Supplementary Datas 2 and 3).
This fact suggests that structural disordering associated with the decrease in crystallinity may be most strongly induced at this mm fraction.
This class includes 14 out of the 20 genes most strongly induced by an elicitor (invertase) in M. truncatula suspension cells [58].
Interestingly, a gene described as LRF, a negative regulator of unfolded protein response [29], was most strongly induced in BT474 cells.
Genes that were widely expressed (several tissues) at relatively high levels (Figure 7A) and most strongly induced by stress (salinity, drought and cold) (Figure 7B) are Os10g34540 (AtTPR1), Os08g41390 (AtROs05g11550g11550 (andP5), and Os02g43020 (AtHop).
Noticeably, the clO hemolysin, the cytK cytotoxin and the enterotoxins hblC, hbLD, hblA and nheA, nheB, nheC, were the genes most strongly induced by PlcR with relative expression ratios of 10 to 50 in the ΔplcR mutant versus wildtype cells.
The most strongly induced genes, LHCR6, LHCR8, LHCX2 and LHCX3, increased dramatically after exposure to HL and were up-regulated as much as 13-36 times already after 0.5 h.
In this analysis, we included the most strongly induced genes for both cytokines (MX1; Table 3) and additionally analyzed expression of OAS1, coding for another important antiviral protein (2',5'-OAS) regulated by IL-28A and IL-29 (32.2- and 41.0-fold, respectively; Table 3).
