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Based on these observations, we screened the next pool of mutants in the most stringent vector GFPi 9/8_FR/FR.
The mutation N54D occurred only after the additional rounds in the most stringent vector GFPi 9/8_FR/FR at 37°C, and is likely the key to the improved solubility relative to the first rounds in GFPi 9/8_FR/SF reporter (Fig. S5).
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This highlights the need to carefully evaluate the optimal Ad vector in the most stringent available preclinical model.
We evaluated this class of vectors in various established breast cancer cell lines, primary breast cancer cells and finally in the most stringent preclinical available substrate system, constituted by precision cut tissue slices of human breast cancer and liver.
These limits are the most stringent to date.
"Best" efforts is the most stringent standard.
Stand-off analysis demands the most stringent requirements on instrumentation.
These are the most stringent collider limits to date for scalar mediators, and the most stringent for pseudoscalar mediators at low masses.
The changes are among the most stringent in the country.
"We abide by the most stringent rules," he said.
Media companies have taken the most stringent steps so far.
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CEO of Professional Science Editing for Scientists @ prosciediting.com