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In this study, the cells were exposed to PMMTM, PMCON, or left untreated for 3 months, after which they were grown in complete medium (without treatment) for at least 10 passages and examined for anchorage-independent growth by soft-agar colony formation assay, which is one of the most stringent indicators of neoplastic transformation.
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Colony formation under soft agar assay, the gold standard test to evaluate the ability of cells to undergo anchorage-independent growth, is therefore the most stringent indicator for neoplastic transformation.
"Best" efforts is the most stringent standard.
"We abide by the most stringent rules," he said.
Media companies have taken the most stringent steps so far.
The changes are among the most stringent in the country.
The state law is considered one of the most stringent in the country.
He said the restrictions on sulfur dioxide will be the most stringent in the nation.
He was placed under the most stringent form of probation, house arrest.
One of its most stringent articles forbids communication with the media..
Renaissance put the industry's most stringent cancellation policy into effect in February 1999.
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