Sentence examples for most stringent assay from inspiring English sources

It is worth mentioning that the most stringent assay applied in the present study is the in vitro differentiation assay that corresponds to the teratoma-formation assay in vivo.

This assay is used to measure the ability of cells to grow in soft agar in an anchorage-independent manner, which is considered the most stringent assay for detecting malignant transformation of cells.

As most stringent assays in murine systems chimaera formation and tetraploid complementation are commonly accepted.

Taken together, these results indicate that mRNA-derived iPSC clones from multiple independent fibroblast lines, with and without addition of VASA are fully reprogrammed by the most stringent assays available to date for human pluripotent cells (17).

For human cells the teratoma assay and in vitro differentiation assay are the most stringent pluripotency assays applicable.

The most stringent pluripotency assay, tetraploid embryo complementation, demonstrated that mouse iPSCs can give rise to all tissues of the embryo proper [ 85, 86].

The formation of a teratoma (a benign tumour comprising cell types representative of each of the three embryonic germ layers) at the site of implantation is the most stringent validation assay available for the differentiation potential of putative hPSCs [ 18].

In mice, only small proportions of these cells are fully reprogrammed based on the most stringent tetraploid complementation assay for evaluating pluripotency [10 13].

Collectively, our results establish that stable knockdown of miR-126 expanded mouse and human long-term repopulating HSC, assessed by using the most stringent phenotypic and functional assays available.

It is noteworthy that the bivalent genes profiles of the iPSC lines described to contribute to viable mice through tetraploid complementation assay (the most stringent proof of pluripotency available so far, GSE16925 and GSE17004) have the highest correlation coefficients when compared with the ESC lines.

To date, the T7 RNA polymerase assay represents the most stringent test of the steric occlusion model for several reasons.