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The data reveal that the common element among the three most stimulatory codons is the potential to form a stable GC base pair at the second position.
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No inhibitory clones affected the expression of the reporter gene to an extent comparable to that of the 5 most stimulatory clones (Fig. 3a).
The dose that showed the most stimulatory effect was chosen for further study.
The activation of platelets by most stimulatory agents leads to an increase in the concentration of cytosolic calcium (Ca2+).
The GACGTT hexamer is the most stimulatory CpG motif in rodents, whereas GTCGTT motif works best in primates.
We found that most putative optimal codons (9 10 codons) occur coincidently in three samples.
However, it should be noted that most putative optimal codons (9 10 codons) occurred coincidently in three samples.
For example, the most frequently used codons are UUU F) (63 codons per 1000 codons), followed by AUU I) (54 codons per 1000 codons) and then AUA(M) (49 codons per 1000 codons).
As in other metazoans, anti-codons occasionally diverge from the most commonly used codons in degenerate codon families.
For example, the most frequently used codons are TTT (F) (105 codons per 1000 codons) and TTA (L2) (78 codons per 1000 codons).
OptZGP was further codon optimized to 70% by choosing the most abundant and/or second most abundant codons.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com