Again, the most stable reference gene was TUBA.
Selection of the most stable reference gene is critical for a reliable interpretation of gene expression data using RT-PCR.
For example, the most stable reference gene for Talaromyces marneffei was GAPDH, followed by TUBA, and ACTB (Dankai et al. 2015).
In the separate assessments, the most stable reference gene was the same, but the other genes were ranked differently in the different analyses.
Application of the ∆Ct approach pointed to LOC_Os03g13170 as the most stable reference gene, in accordance with the results from geNorm (data not shown).
Interrelated analysis provided by BestKeeper concluded that the most stable reference gene was EF1, and TUBA was the second most stable.
However, the most stable reference gene was the same (TUBA), followed by EF1, EF2, RPS5, RPS24, ACTB, UFD, UBC and 18S.
The aim of this study was to identify the most stable reference genes in Alternaria sp. under different growth conditions and resveratrol production conditions.
In this approach, the most stable reference gene was identified by the comparison of SD value and CV value of these selected genes.
The comprehensive results of this research demonstrated that TUBA and EF1 were the most stable reference genes, 18S was the least stable gene, and the other candidate reference genes were intermediate among all six sets of experiments.
As shown in the results of GeNorm analysis (Fig. 2), UBC and Rps24 showed the lowest M values, indicating that they were the most stable reference genes used for measurement.
