Sentence examples for most stable internal from inspiring English sources

Furthermore, it is considered the most stable internal control in endurance exercise studies [ 23, 24].

RNU6B was found to be the most stable internal reference miRNA across treatments with an M value of 0.7.

In these cases, a method for normalization to two or more of the most stable internal control genes identified might be necessary.

We then identify the most stable internal reference genes using the geNorm, NormFinder and BestKeeper algorithms and present guidelines for transcript analysis in different tissues of E. coca by qRT-PCR.

The HPRT1 gene was used as an endogenous control after evaluating different housekeeping genes in several breast cancer cell lines and identifying HPRT as the most stable internal control.

Gene expression levels were normalized using the geometric mean of the 4 most stable internal control genes in NB (i.e. UBC, HPRT1, SDHA and GAPD) as reported previously [ 40].

The most stable pair of internal controls was a combinations of two genes, U6 and U91 (stability value = 0.036; Table  5).

P values of the Student's t-test for the significant differences are shown in bold NormFinder algorithm was used to calculate the most stable pairing of internal controls.

To choose the most stable genes as internal references for RT-qPCR data normalization, four candidates [ β-actin (β-act), ribosomal protein L13a (rpl13a), β-2-microglobulin (β2 mg) and elongation factor-1α (ef1α)] were selected according to their expression levels detected in the microarray studied.

RT-qPCR data (threshold cycles) were linearized, and the NormFinder algorithm was used to calculate the most stable gene among the internal controls.

Real-time PCR was carried out for eleven housekeeping genes in order to select the most stable gene as the internal control for all the compared tissues (HbelF1Aa, HbUBC4, HbUBC2b, HbYLS8, HbRH2b, HbRH8, HbUBC2a, HbalphaTub, Hb40S, HbUbi, HbActin).