Sentence examples for most stable gene from inspiring English sources
Therefore, BestKeeper is highly recommendable to select the most stable gene to be used as internal standard and the selection of a specific reference expression gene requires a validation for each tissue and experimental design.
The best single reference gene was not included in the most stable gene combinations (Table 1).
In addition NormFinder algorithm identifier gpd as most stable gene (Additional file 1: Figure S3).
Hence, the selection of the most stable gene or set of genes under the particular experimental conditions as well as analytical approach is crucial to accurate profiling of gene expression.
However in CMCs, GAPDH housekeeping gene emerged as the most stable gene with no closely comparable housekeeping gene among the top 4 evaluated genes (range, 433.8 stability units).
When we investigated the most stable gene, Rps13, we observed up to 6-fold expression differences across tissues in our dataset.
A set of three most stable genes is found sufficient to be used as housekeeping genes for lung tissues in studies of similar design.
geNorm ranked 28S rRNA/TUB as the most stable genes in brain, heart and spleen (Fig. 2a c).
The GeNorm algorithm identified gpd and act as the most stable genes along all the conditions assayed, displaying an expression stability value (M-value) of 0.213.
The results of BestKeeper analysis showed that in brain, heart and head kidney, 28S rRNA was ranked as highly stable RGs, while in liver, muscle and spleen, respectively, RPL30, TUB and ELF-1A were the most stable genes (Table 5).
Based on the least M value the most stable genes in brain, heart, liver, muscle, spleen and head kidney were RPL30/28S rRNA, 28S rRNA/TUB, 28S rRNA/B2M, ELF-1A/TUB, ELF-1A/28S rRNA and ELF-1A/28S rRNA, respectively (Fig. 3a f).
