Sentence examples for most stable expression from inspiring English sources
Stepwise exclusion of the gene with the highest M value results in a combination of two constitutively expressed reference genes that have the most stable expression in the tested samples.
The most stable expression genes had the lowest M value, and vice versa.
The Mi-actin gene showed the most stable expression pattern (lowest geometric mean) and was used to further normalize Mi-crt expression levels.
Gene stability analysis via geNorm software uncovered MRPL39, GPR175, UXT, and EIF3K as having the most stable expression ratio and, thus, suitable as ICG.
It was located on the qGS3.2 region in our QTL analysis, which was recurrent across four environments for GL, LWR, CS, PL and AS, showing the most stable expression and pleiotropic effects (Fan et al. 2006).
As described above, we first determined the housekeeping gene that had the most stable expression in a given cell type upon IFN or IC treatment in order to normalize our results.
Also in mouse expression arrays genes encoding for ribosomal proteins are the most stable expressed ones.
The lowest stability value represents the most stable gene expression within the gene set examined.
These relative quantities were then entered into a data input file which could be analyzed by the geNorm software package to identify which of the assayed genes for a given tissue type exhibited the most stable relative expression.
Unsupervised hierarchical clustering (HCL; Partek Genomics Suite, v6.6) of gene expression and methylation data were, respectively, established using Pearson's Dissimilarity performed iteratively on 200 2,000 genes and 200 10,000 probes to identify a minimal gene set associated with the most stable gene expression and methylation tumor cluster patterns (Supplemental Fig. 2).
This process insured that the final normalization was based on the most stable gene expression measurements across the arrays and that the probesets on the U133 Plus arrays, but not represented on the U133A arrays, did not influence the final normalized values.
