Your English writing platform
Discover LudwigSuggestions(1)
Exact(1)
We performed normalization for each array using the most stable controls.
Similar(59)
The most stable control genes (lowest variation in mRNA expression) have the lowest M value.
The most stable control genes were determined by geNorm among five potential genes.
The most stable control genes for normalization of the relative concentrations of mRNA were identified using the GeNorm software [ 29].
Control microRNAs RNU48 and miR-423-3p miR-423-3p miR-423-3plity over all tumors and miR-423-3p wereselectested the most stable control.
Expression values were normalized by a factor calculated from the three most stable control genes for the cell lines and tumours, (Table 2).
ACTB and GAPDH were selected as the most stable control genes from four endogenous genes tested (GAPDH, ACTB, HPRT1 and BCR) using the geNorm algorithm.
The two most stable control genes, GAPDH and HPRT, were used by qBase to normalise the expression values of all the replication and repair targets.
These tested genes were analyzed for the most stable control gene with the lowest expression stability, as defined as an internal control that was considered to be most stable under the tested experimental conditions.
Its calculations use the geometric mean of the expression of the candidate genes and by elimination of the least stable control genes the two most stable control genes are identified.
Initial steep decrease in average M value of each gene is shown in Additional file 8, which firmly demonstrates that β-Actin is the most stable control gene (with the lowest M value).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com