Sentence examples for most stable control from inspiring English sources
The most stable control genes (lowest variation in mRNA expression) have the lowest M value.
The most stable control genes were determined by geNorm among five potential genes.
The most stable control genes for normalization of the relative concentrations of mRNA were identified using the GeNorm software [ 29].
Expression values were normalized by a factor calculated from the three most stable control genes for the cell lines and tumours, (Table 2).
Control microRNAs RNU48 and miR-423-3p miR-423-3p miR-423-3plity over all tumors and miR-423-3p wereselectested the most stable control.
ACTB and GAPDH were selected as the most stable control genes from four endogenous genes tested (GAPDH, ACTB, HPRT1 and BCR) using the geNorm algorithm.
We performed normalization for each array using the most stable controls.
geNorm software was used to establish the two most stable internal control genes (MRPL19 and PUM1) from a group of four endogenous controls (ACTIN, GAPDH, PUM1 and MRPL19), followed by the calculation of the normalization factor for each tissue sample (Table S2 in Additional file 1).
All the reactions were carried out in duplicate, and fold changes in gene expression were determined by using the formula 2-ΔΔ C t. geNorm software [ 32] was used to establish the two most stable internal control genes (MRPL19 and PUM1) from a group of four endogenous controls (ACTIN, GAPDH, PUM1 and MRPL19), followed by the calculation of the normalization factor for each tissue sample.
Control genes TBP and CREB1 were tested for stability over all tumors, and TBP selected as the most stable endogenous control.
The HPRT1 gene was used as an endogenous control after evaluating different housekeeping genes in several breast cancer cell lines and identifying HPRT as the most stable internal control.
