Your English writing platform
Discover LudwigSuggestions(5)
Exact(6)
Pair wise comparisons using geNorm revealed that HPRT and RPS23 were the most stable combination of IRGs with M-value of 0.29 followed by UXT (0.30) and RPL4 (0.31).
For normalization of constitutive heat shock gene expression, the control animals were tested separately, and Grp78 and β-actin were rated as the most stable combination.
Normalization factors per sample were calculated using the geometric mean of the most stable combination of these normalization assays, determined by the measure of their pairwise variation as calculated by geNorm[ 14].
5S and U6 were excluded from the set of reference genes suggested by geNorm; the most stable combination based on the geNorm analysis included miR-221, miR-103, let-7a, miR-181c, miR-181a, and miR-26a (RG-6).
The typical methodological approach used in these studies has been to test a selection of commonly used reference genes such as GAPDH, β-actin, α-tubulin, hypoxanthine phosphoribosyl-transferase 1 and 18S RNA to identify the most stable combination specific to the sample and experimental conditions under investigation [ 23- 29].
In case of normalization by RPL29 which was predicted as most stable single reference gene in NormFinder and most stable combination with HPRT1 in geNorm, the high-low pattern of RQ difference between normal and tumor was similar to RQ by HPRT1 though there were difference in three patients.
Similar(54)
The most stable combinations of miRNA and the target gene or MIRNA genes were normalized to percent minimum free energy which works well for plant miRNAs because plants possess near perfect complementarity between miRNA and target genes [65], [115].
For simplicity, we adopted the most stable combinations of similarities provided in [ 14].
It was also seen that endcaps affected which tube combinations were more stable; the armchair@armchair DWCNT was the most energetically stable combination for capped tubes, while the armchair@zigzag DWCNT had the highest stability of uncapped tubes.
The best single reference gene was not included in the most stable gene combinations (Table 1).
GeNorm [ 18] was used to select the most stable pair-wise combination of reference genes, which were PGK1 and GAPDH.
More suggestions(15)
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com