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Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) is an inexpensive, time-saving genotyping method that is applicable for most single nucleotide polymorphisms.
The open reading frame with the most single nucleotide polymorphisms encodes the regulatory protein IE62, but IE62 studies have failed to define a specific polymorphism associated with attenuation.
If Skts5 is representative of the genome as a whole, then most single nucleotide polymorphism data available for Spret/EiJ from coding regions will have utility for other M. spretus strains.
While most single nucleotide polymorphisms (SNPs) are di-allelic and easier to detect, many SVs are multi-allelic in general and their patterns vary significantly among different SV types [ 7].
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However, the biological significance of most single-nucleotide polymorphisms (SNPs) in noncoding regions remains to be elucidated [ 26– 28].
The complete story of the MnSOD genotype is probably quite complex, a situation that has proven true for many or most single-nucleotide polymorphisms [ 42, 43].
In addition, it is also very useful for discovery of new associations when applied to GWAS, because each study provides information for most single-nucleotide polymorphisms (SNPs) in the genome, either directly or through imputation, and therefore add to the overall result (17).
Among these mutants, most were single nucleotide or short fragment-indels, while long fragment-inserts derived from the backbone of the gRNA vector were also observed.
Analysis of the four most common single nucleotide polymorphisms (SNPs) found in the CRP gene that were associated with increased circulating CRP (95% CI of 58-87% increase) revealed that there was no increase in cancer risk or incidence associated with these SNPs[99].
Based on pairwise linkage disequilibrium, haplotype block structures, and structure of genes, we identified the 14 most informative single nucleotide polymorphisms in the region (Table 2).
Replication of association with LDL-C level was sought for the most significant single nucleotide polymorphism (SNP) within the LDLR gene in three European samples comprising 6 642 adults and 533 children.
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