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The most similar sequence (85.3% similarity) from a cultivated isolate came from Methylophaga marina.
For each sequence, the most similar sequence (with a similarity score E-50 at least) in the database was recorded.
For ascomycete fungi (the group to which most of our endophytes belong; see below) we used Phyutility [5] to determine the similarity of each GenBank nrDNA sequence to the most similar sequence in GenBank (Fig. 1B).
Subsequently, all candidate sequences are compared with each upstream sequence of all genes to identify the most similar sequence in each upstream sequence.
The most similar sequence that we could find is AKNCQ, present in the D1 dopamine receptor.
Sequence data were aligned to the most similar sequence using the BLAST database algorithm [100], and then further analysed with the ARB software package [102].
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Protein sequences of the genes spanning from Phvul.002G200900.1 to Phvul.002G202200.1 on the bean genome sequence were used as query in Bl2seq to detect most similar sequences in the sequenced genomes of G. max, C. arietinum and M. truncatula.
In single linkage, it is the similarity between the two most similar sequences; in complete linkage, between the two most dissimilar sequences; and in average linkage, the average of all pairwise similarities.
Although the capsid protein of SsHADV-1 lacks sequence similarity with those of any geminiviruses, its most similar sequences are commonly found in environmental samples.
We recorded species designations of the first three most similar sequences, which in all cases showed a similarity of more than 99.1% (average BLAST bitscore of 1095 ± 4.31 SEM).
A BLAST (http://blast.ncbi.nlm.nih.gov/Blast.cgi) search retrieved such highly similar genes only from Y. pestis sequences; the next most similar sequences of other Enterobacteriaceae were at 78% similarity.
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