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One of the most significant network motifs is the coherent feedforward loop, in which transcription factor X regulates transcription factor Y, and both jointly regulate gene Z.
Ms. Drnovsek-Zorko said that she had signed "out of civic carelessness" and that it was her conviction that ACTA "limits and withholds the freedom of engagement on the largest and most significant network in human history".
"Quite simply, I did not clearly connect the agreement I had been instructed to sign with the agreement that, according to my own civic conviction, limits and withholds the freedom of engagement on the largest and most significant network in human history, and thus limits particularly the future of our children". She urged opponents of Acta to attend the protest in Ljubljana "in my name".
We focus here on the most significant network.
The most significant network highlighted cardiovascular disease and molecular transport (Figure 5A), consistent with a genetic component influencing circadian BP variation and thereby cardiovascular disease in this model.
In addition the present study focuses on the most significant network modules identified, however some of the proteins identified in [24] were found among less significant modules identified in the present study (data not shown).
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Tropoelastin appears in the most significant networks at day 3 and 7, but not at day 25.
The top four most significant networks identified from the Singh dataset (Figure S5) represent genes connected to the same cytokines and growth factors identified in the Lapointe dataset.
The network analysis was performed independently in the two datasets and the most significant networks (statistically significant and with >50% target genes represented in the network) were selected for further analysis.
One of the most significant networks that emerged from the TNFα induced altered genes was that of lipid metabolism and this subsequently correlated well with the top canonical pathway, the biosynthesis of steroids.
Lists of genes resulting from analyses were submitted to Ingenuity Pathway Analysis (IPA; Ingenuity® Systems, Redwood City, Ca, www.ingenuity.com) in order to obtain the most significant networks and canonical pathways related.
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