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The SSEARCH results were sorted according to their statistical significance (E-value), with the most significant hits on top.
Among the down-regulated transcripts, the most significant hits included integrin cell surface interactions and negative regulation of cell proliferation; among the up-regulated transcripts, the most significant hits included nucleotide synthesis, cell cycle checkpoints, and RNA processing.
The 95 year old actor, producer and writer has presumably expanded on earlier tellings of the making of one of his most significant hits.
Searches seeded with the nucleotide sequence of BMV2 resulted in the most significant hits.
The top 20 most significant hits are shown in Table 3.
Therefore, filtering the segments characterised by high and wide peaks on the scoring profile selects the most significant hits.
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A SNP cluster tagged by rs1006417 on chromosome 14 was the most significant hit (P = 1.4 × 10−6), located in a gene poor region with no known genes (Additional file 1: Figure S3).
The DCN of the species of the most significant hit of a protein is used to predict is function.
In these studies, the most significant hit (rs11136000) is located in an intron of CLU, on chromosome 8 [4], [4].
If protein PA in species A picks up protein PB in species B as the most significant hit, it is not necessary that protein PB pick up PA in species A. This asymmetry could be used to restrict the orthology requirement to symmetric best-best hits [5].
In addition, the EST libraries of a non-insect arthropod, the Arctic springtail Onychiurus arcticus, contain an EST clone (GenBank Accession No. EW749693.1) encoding a partial protein sequence in which the C-terminal (aa 68 218) displayed high similarity to TMS domains of CatSper4, with N. vectensis CatSper4 as the most significant hit (E-value, 3×e−27).
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