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We identified a group of 1419 genes using a q-value (the adjusted p-value found using an optimised FDR approach) of 5% to retain the genes with the most significant differential expression (Fig. 2).
A list of putative anchor genes were selected as those displaying; a) the most significant differential expression ratio (>2 x median expression fold change), b) high raw signal intensity (>100 RFU) above background and c) low median signal intensity in all other subcompartments examined (Figure 1).
To identify differentially expressed glomerular genes, we searched for common genes between glombase [ 42], SAGE glomerular tags [ 43] and 220 annotated transcripts with the most significant differential expression between ROP- Os/+ versus C57- Os/+ kidneys.
Furthermore, samples collected within the first 4 days since transplant surgery in both patient groups showed the most significant differential gene expression, potentially reflecting the heavier immunosuppressive induction therapy provided to patients immediately after surgery.
Interestingly, the gene displaying the most significant differential expression in this study, which demonstrated fourfold higher expression in resistant birds (p adj = 5.6e−20), encodes an epithelial calcium-activated chloride channel (LOC424523)(Connell et al. 2012).
For our LDA analyses, we considered as a starting point the 318 genes selected from a previous work (Bermudo et al, 2008) as those with the most significant differential expression between benign and adenocarcinoma prostate tissue samples.
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These genes were among the 10 genes that showed the most statistically significant differential expression between ES cells and differentiating cells.
We selected some of the most significant and markedly differential genes to measure their expression levels using a quantitative PCR approach.
Therefore, most important differential diagnosis was rhabdomyosarcoma.
The first is to manage each lesion with therapy designed to be adequate for management of the most significant consideration in the differential diagnosis.
The most significant promoter associated with differential methylation was the ferritin light chain (FTL) gene.
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