Sentence examples for most significant alignment from inspiring English sources
Using the blastn algorithm of BLAST+ 2.2.30 [ 36, 37] and the command line options -db nt, -max_target_seqs 1, -outfmt "6 qseqid sseqid staxids sscinames pident length mismatch gapopen value", each DNA and RNA contig was aligned to the NCBI non-redundant nucleotide database and the most significant alignment was returned.
A combination of protein sequence alignment, and the correlation score with the subtype that gave most significant alignment with other subtypes was used to identify invariant regions.
This method not only gave the most significant alignment result, but also eliminated differences arising from the variable number of available sequences of different subtypes and such an approach can be useful for other viruses with high rates of mutation.
The most significant alignment was then removed, and the second most significant alignment was combined with the remaining query-template alignments in order to generate a multiple sequence alignment using the same algorithm.
bName: This output only contains descriptions of the top blast hit (most significant alignment based on E value).
The minimal domain compactness between query and target proteins was calculated between the domain pair with the most significant alignment.
The analysis was performed in two steps; first, individual profiles of each subtype were generated and each profile was then aligned to the H2 profile to obtain the most significant alignments.
The alignment scorecard gives information regarding the extent of homology to each of the test species for the top scoring alignment, and the second and third most significant alignments.
Because the profile for H2 had the most significant alignments to the other profiles, as measured by COMPASS E-values, it was used to create a seeded multiple alignment for the profiles similar to a PSI-BLAST [13] alignment.
As E-value and z-score estimated by the WUBLAST, FASTA and SSEARCH programs depend on sequence length and the size of search database, we assessed the significance of alignments by P-value calculated in the PRSS program from FASTA packed and selected the most significant alignments.
Only the two most significant alignments were retained in an attempt to avoid spurious alignment to multiple genes from different stickleback linkage groups (for example due to similarity of genes from the same gene family).
