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In four exocyst mutant lines with the most severe root growth defects (exo84b-1, sec8-3, sec8-4, and sec8-4 exo70A1), we predicted a more profound alteration in the distribution/pattern of DR5 expression if the exocyst mutant's slower root growth was due to a defect in auxin response in the stem cell niche or MZ.
And indeed, exocyst mutants with the most severe root growth defects, sec8-3 and exo84b-1, demonstrated a mild rescue when grown on media containing low concentrations (1 or 3.16 nM) epi-brassinolide, whereas wild-type Col-0 plantshoweded a decreased growth rate in this same concentration range, and the brassinosteroid receptor mutant, bri1, showed no response (insensitivity).
Second, although the dynamics of BRI1 cycling is altered from wild-type in exocyst mutants treated with BFA [ 36], the ultimate localization of BRI1-GFP to the plasma membrane is not significantly altered in exocyst mutants with the most severe root growth defects (Additional file 1: Figure S9).
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In the presence of 1 μM 24-epiBL, the length of WT roots was reduced to 22% of roots grown in the absence of 24-epiBL, whereas roots of the most severe OCP lines were reduced to only 65%to75%5% relative to those grown without exogenous 24-epiBL.
Among overexpressing lines, the ten plants with the most severe phenotypes did not develop any crown roots, but the radicles and lateral roots appeared normal.
Multiple-rooted teeth with different periapical statuses at different roots were classified according to the most severe periapical condition.
Deafferentation of the contralateral forelimb by dorsal root section in injured animals resulted in the most severe and long-lasting tactile and sensorimotor deficits.
Even in lines with the most severe defects, the organization of the quiescent center and tissue layers at the root tips appears similar to wild-type, although meristematic, transition, and elongation zones are shorter.
In contrast, rate of cell elongation is reduced in parallel with growth rate only for the exocyst mutants with less severe root growth defects; whereas the three exocyst mutant lines with the most severe defects in root growth (sec8-3, sec8-4 exo70and and exo84b) share the same dramatically reduced cell elongation rate.
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