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A variety of reactive protein bands were revealed; most sera bound to proteins with an approximate mass of 210 kDa (35, 34% of sera) and/or 75-55 kDa (39, 37.9% of sera).
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Again some of the SNMG sera bound weakly to the unclustered AChR but most SNMG sera showed significantly more (P < 0.01) binding to clustered AChR than to unclustered AChR (Fig. 3B).
The immune sera bound to both synthetic mannose oligosaccharides and gp120 proteins from a broad range of HIV-1 strains.
Six sera bound all three chimeras indicating that they contained multiple IA-2 specific PTP domain antibodies.
As expected, all the acute sera bound to fewer spots than their paired convalescent sera.
AS sera bound 112 proteins, SLE sera bound 91 proteins, SSc sera bound 103 proteins and healthy controls' sera bound 89 proteins (data not shown).
Not all individual positive sera bound significantly better than all four individual negative sera.
Both lamprey plasma and mouse sera bound HA and NA.
Five sera bound to contactin-2 itself but four of these sera also bound to Kv1, Caspr2 or Lgi1.
9 out of 10 positive sera bound better to the antigen putative dihydroxyacid dehydratase compared to the negative sera.
7 out of 10 positive sera bound better to the antigen 2,4-dieonyl-CoA-reductase compared to the negative sera.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com