The Belgian serogroup 23 colonizing pneumococci present most likely sequence variability in the target gene (wzy for serotype 23A and 23F, wzx for serotype 23B) and are therefore not amplified by the current primer pairs.
They are the most abundant class of sequence variability in the genome, co-dominantly inherited, easily automated and, thus, appropriate for high throughput analyses [ 11].
With regard to point (a), little experimental data is available for most kinases with regard to the sequence variability of substrates, structural detail of kinase-substrate complexes, kinetic or energetic aspects of the interaction.
It exhibits a high degree of sequence variability in the most structurally conserved region of the HIV-1 envelope [ 22, 23] and it interacts with the co-receptors CCR5 and CXCR4 [ 7, 24, 25].
By combining the criteria of energy stabilization and sequence variability, we selected nine most promising mutations (Table 2).
DGGE analyses were undertaken to assess the intragenic sequence variability of two of the most prevalent AR genes, tet(W) and tet(M).
Re-sequencing genomic regions of randomly selected miRNAs and 3'UTRs with SNPs using Sanger sequencing revealed that most SNPs were successfully detected, indicating that the sequence variability was mainly from SNPs in genomic DNA.
This immune-driven sequence variability in one of the most prominent virulence factors of T. denticola might underlie the pathogenic mechanisms that govern the development of periodontal diseases in vivo.
However, most of these applications either targeted viruses with relatively little sequence variability, such as severe acute respiratory syndrome virus [29], or only selected regions of viruses having larger sequence variability [43] [45].
