Apart from a role in subunit assembly, no clear function has been ascribed to this large and most sequence-diverse domain (Hansen et al., 2010; Kumar and Mayer, 2013), although deletion of the NTD slows desensitization kinetics (Bedoukian et al., 2006; Möykkynen et al., 2014; Pasternack et al., 2002).
However, it should not be assumed that any single primer pair is capable of amplifying all, or even most sequences representative of diverse microbial populations.
Most sequencing techniques require days of work.
However, most sequences are shorter.
Most sequences started at aa 2284.
When all 4,168 sequences annotated for "catalytic activities" were combined, they represented together with the sequences annotated for "binding activity" (4,027 sequences) the most diverse subsets within the molecular function group.
In terms of our general results, elephants and the manatee were found to contain the most diverse sequences, while hyrax showed comparatively less diversity.
For the generation of phylogenetic trees, the most diverse sequences of each subgroup were aligned with ClustalW [ 18].
In contrast, Group III encompasses the most diverse sequences, probably due more than one U3 version being represented.
We attempted to establish this by using the N-terminal region (which is clearly shared between all dystrophins, dystrobrevins and dystrotelins) to perform a phylogenetic analysis against our most diverse sequences.
Indeed, lineage IV is the most geographically diverse, with sequences from different continents and sub-continents: India, China, Near-East and Africa.
