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The most sensitive strains are hfi1Δ and spt20Δ, displaying sensitivity at 25 μM CG-1521.
We found that loss of a large number of TA proteins (Δpep12, Δtlg2, Δsec22, Δvam3, Δscs2, Δsso2, Δgos1, and bos1-DAmP) caused pronounced copper sensitivity (see Figure 5 for the most sensitive strains).
The most sensitive strains to these compounds were LL01 8.3.8 and LL01 8.3.1, being compound 11 up to 20 times more active than enrofloxacin.
This proves that the killing effects of many tested compounds could be expected on the most sensitive strains [35].
Among the most sensitive strains, several are involved in ubiquinone synthesis.
Color mutant #5 was one of the most sensitive strains in the Af293 background tested, which is consistent with our observations that #5 produced white conidia and bears a mutation in the alb1 gene.
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In that connection, it should be noted that although mouse strain-related differential sensitivity was reported for AOM-induced carcinogenesis, with A/J being the most sensitive strain, a strain difference between B6 and A/J mice was not observed in AOM-induced DNA adduct formation (data not shown).
The most sensitive strain in both paradigms, F344, was chosen for further pharmacological validation.
Additional studies in congenic BALB/c, nude, and beige/Scid mice demonstrated dose-dependent hepatotoxicity and indicated that beige/Scid was the most sensitive strain.
The most sensitive strain, i.e., P. aeruginosa 3, was selected to examine the effect of low concentrations (100 to 10 ppm) of lyso-Ag NPs (Figure 5).
P. aeruginosa 3 was the most sensitive strain as indicated by the formation of maximum zones of inhibition (mm) in the presence of all four types of lyso-Ag NPs applied.
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