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The most sensitive molecular processes - for example, enzyme and receptor biochemistry [3, 4] - operate at submicromolar physiologic conditions, often as low as the nanomolar or picomolar range.
The most sensitive molecular imaging modalities are SPECT (~10−10 mol/L) and PET (10−10 10−12 mol/L) using radiotracers (Fani and Maecke 2012; Fani et al. 2012; Gaertner et al. 2012).
Even with the most sensitive molecular methodologies, yield was shown to be strongly related to the time since onset of symptoms [9], [14], with a 3-fold decline in proportion positive within 3 to 5 days after onset of symptoms for both RT-PCR and culture procedures.
One of the most sensitive molecular targets of PCBs is RyR activation (Pessah and Wong 2001).
The most sensitive molecular marker was NY-BR-1 being positive in 13 SLNs, followed by MGB-2 in 12 SLNs and CK-19 in eight.
RT-PCR seems to be the easiest and most sensitive molecular method for detection of the BCR- ABL transcript in both these situations.
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As the most sensitive criterion, major molecular response defines absence of a specific disease-associated mutation in previously positive cases [ 64].
Future development of these techniques, especially on the most sensitive PCR-based molecular techniques, will certainly yield a more informative picture about anammox bacteria in the natural ecosystem.
We found the most sensitive and reliable molecular indicator of dioxin-induced diabetes to be the ratio of mRNA of glucose transporter 4 (GLUT4) and nuclear transcription factor kappa B (NFκB), a marker of inflammation.
Based on previous comparative studies [ 24], kDNA PCR was expected to be the most sensitive genus-specific molecular technique, which may explain the positive rates of kDNA amplification from the DNA extracted from the blood samples of the four dogs and the two negative samples in the hsp70 assay, which is less sensitive.
Lipid bilayers of biological membranes appear one of the most pressure-sensitive molecular assemblages (Wann & Macdonald, 1980; DeLong & Yayanos, 1985; Somero, 1992; Macdonald, 1997; Winter & Dzwolak, 2005); pressure increase orders structures and reduces flexibility in lipids, nucleic acids and carbohydrates (Behan et al., 1992; Balny, Masson & Heremans, 2002).
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